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DNA extraction from the greater celandine (or tetterwort), Chelidonium majus

Chelidonium majus, commonly known as the greater celandine or tetterwort (in America, the latter refers to Sanguinaria canadensis), is the only species in the genus Chelidonium, family Papaveraceae. The lesser celandine is not closely related, but its family, the Ranunculaceae, is allied to the Papaveraceae (Order Ranunculales). The greater celandine is native to Europe and the Mediterranean basin. It is also widespread in North America, having been brought there by settlers as a herbal remedy for skin problems such as warts as early as 1672.

How to do it

0.087 g leaf were homogenized in 250 ul Mole Plant lysis buffer using a Precellys 24 homogenizer (Bertin technologies) with CK14 kit and the 6500 - 2x20 protocol. Incubation at 65 ="EN-US">°C for at least 10 minutes followed by 10 min centrifugation at 4000rpm. 200 ul of the supernatant were used as input in the GeneMole, using the MoleStrips DNA Plant and the DNA plant 8 protocol.